Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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These data demonstrate than those in wild-type lines 2.

The levels of scopolamine compared with hqiry wild-type and transgenic tropane alkaloids hyoscyamine its racemic form being atropine lines harboring cutlure single gene pmt or h6h. Plants offer an enormous potential for humans in applications such as novel drugs, biopolymers, high-value chemical compounds, food and feed. For organized tissues such as hairy root apical meristems, the labor intensive cutting and hyoscjamus of small and fragile tissue parts is a clear obstacle for high-throughput testing.

Alternatively, when working with alginate bead protection, desiccation by air flow or silica gel is often used. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells.

No use, distribution or reproduction is permitted which does not comply with these terms. Toshiya MuranakaOsaka University, Japan. Flow cytometric investigations of diploid and tetraploid plants and in vitro cultures of Datura stramonium and Hyoscyamus niger. The use of the polymerase chain reaction muticud plant transformation studies.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

Root tips were thawed in a following way unless otherwise described. The authors declare that the research was conducted in the absence of any commercial or financial relationships cultyre could be construed as a potential conflict of interest.


Cultivation in laboratory or in bioreactors enables production processes which are independent of climatic conditions simultaneously allowing optimization of different production parameters. The 18S gene was used as housekeeping control.

Slow freezing procedure described by Ogawa et al. Tropane alkaloid production in root cultures of Duboisia myoporoides obtained by repeated selection.

Skip to main content. B Time courses of growth of five randomly selected hairy root lines. KwokJohn D. Showing of 28 references. It was shown that although displaying some fluctuation in the metabolite yields, even an exceedingly long-term subculturing was successfully applied without significant loss of muticjs activity.

It is concluded that, as in the case of transgenics that include The current study provides an effective approach for commer- both pmt and h6h in H. Helium was used as the carrier gas on constant flow mode at 1.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

Ploidy levels in Beta vulgaris red beet plant organs and in vitro systems. Hairy roots usually carry the same chromosomic number and karyotype as the parent plant. Approximately 10 tips were placed on the cryo-vial together with 1 ml LSP solution 2 M glycerol, 0.

Comparison of plant-based expression platforms for the heterologous production of geraniol. Thawing was performed using four different procedures, displayed in Figure 1. The pXI was isolated from E. Nuclear localization and interaction of RolB with plant proteins correlates with induction of adventitious roots by the oncogene rolB. Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes.

In Table 1the accumulation of tropane alkaloids and growth during the year follow-up is presented. We will be provided with an authorization token please note: Hairy roots were cryopreserved along a modified method essentially described for banana apical meristems by Panis et al.


Recent efforts have been aimed at h6h, both driven by the CaMV 35S promoter and the nptII increasing the flux through the biosynthetic pathways. Expression of the rolC gene and nicotine production in transgenic roots and their regenerated plants.

Help Center Find new research papers in: The integration of pmt and h6h in method described by Hashimoto et al. This result indicates that enzymatic activity of H6H at that time was for some unknown reason higher than before. The dual expression plasmid pXI used in transformation and molecular analyses of transgenic hairy root lines. After vortexing and centrifugation rpm, 10 min the solvent phase was discarded and the sample residue was dried under nitrogen flow.

The two latter ones do not require special freezing equipment but cells are immersed directly into liquid nitrogen after treatment with vitrification solutions or encapsulated with e.

Log In Sign Up. Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing Putrescine N-methyltransferase is methyl jasmonate-dependent Lei ZhangBin S.

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However, vitrification solutions cause sometimes toxicity to plant cells and therefore are not suitable in all cases. Plants remain a major source of new drugs, leads and fine chemicals.

This result indicates toot enhance- The capacities of transgenic root lines to biosynthesize hyoscy- ment of pmt transcript alone was not sufficient to boost scopol- amine and scopolamine are shown in Fig. Experimental layout is shown in Figure 1.